Recombinant Human NT-3(Cat. No.:C079)

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Price：

$150

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10μg

50μg

500μg

1mg

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Description

Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.

Accession

P20783

Known As

Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3

Mol Mass

13.6 KDa

APMol Mass

14 KDa, reducing conditions

Formulation Description

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity-SDS-PAGE

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Purity-SEC-HPLC

Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Bioactivity-ELISA

Immobilized Human TrkB (C-6His)(Cat. No.:C507) at 5 μg/mL (100 μL/well) can bind Human NT-3(Cat. No.:C079)*: Biotinylated by NHS-biotin prior to testing. The EC50 of Human NT-3(Cat. No.:C079)* is 15 ng/mL.(Regularly tested).

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping

The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.

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