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股票简称：近岸蛋白
股票代码：688137
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Recombinant Human CD73 (AAH65937.1, C-6His)(Cat. No.:C446)
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COA
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Size：
10μg
50μg
500μg
1mg
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1
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Description
Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
Accession
AAH65937.1
Known As
5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
Mol Mass
58.76 KDa
APMol Mass
62 KDa, reducing conditions
Formulation Description
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity-BLI
Loaded Anti-Human CD73 mAb-Fc(Cat#NC034) on Protein A Biosensor, can bind Human CD73-His(Cat#C446) with an affinity constant of <10-3 nM as determined in BLI assay. (Regularly tested)
Bioactivity-ELISA
Immobilized Human CD73-His(Cat#C446) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034).The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 3.33 ng/ml. (Regularly tested)
Storage
  • Store at ≤-70°C, stable for 6 months after receipt.
  • Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
  • Please minimize freeze-thaw cycles.
Shipping
  • The product is shipped on dry ice/polar packs.
  • Upon receipt, store it immediately at the temperature listed below.
Background
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
仅供科研或生产使用，不可直接应用于人体。
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