
Isotype

Formulation Description
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Purity-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE.(QC verified) 
Purity-SEC-HPLC
Greater than 95% as determined by SEC-HPLC.(QC verified) 
Bioactivity-ELISA
Immobilized Recombinant Influenza B NP(DRA131)at 1μg/ml (100μl/well) can bind FluB NP Antibody (3C11)(DA089).The ED50 of FluB NP Antibody (3C11)(DA089) is 16.64ng/ml.(Regularly tested) 
Bioactivity-BLI
Loaded FluB NP Antibody(DA089) on AMQ Biosensor, can bind Recombinant Influenza B NP(DRA131) with an affinity constant of <1pM as determined in BLI assay.(Regularly tested) 
Pair recommendations
CAPTURE ANTIBODY | DETECTION ANTIBODY | DA088(clone#8B9) | DA089(clone#3C11) |
Please note that pair recommendations are based on results obtained by our laboratory. Equally good results may be obtained using other pairs and therefore these recommendations are only indicative.

Storage
24 months from manufacturing at ≤-20 °C; 1 month, 2 to 8 °C under sterile conditions.

Shipping
The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.

Background
The influenza B virus is the leading cause of annual influenza epidemics and occasional influenza pandemics. NP of influenza B virus plays a central role in viral replication. As a structural protein with no intrinsic enzymatic activity, it is the most abundant viral protein in infected cells. Monomeric NP is a 56 kDa protein with a patch of basic residues capable of binding to a single-strand RNA. NP also contains nuclear localization signals (NLS) and nuclear export signals (NES). The Influenza B virus nucleoprotein (NP) is the major protein component of the genomic viral ribonucleoprotein (vRNP) complexes, which are the replication- and transcription-competent units of Influenza viruses. Early during infection, NP mediates import of vRNPs into the host cell nucleus where viral replication and transcription take place; also newly synthesized NP molecules are targeted into the nucleus, enabling coreplicational assembly of progeny vRNPs.
For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.
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