Recombinant BRSV(strain BO/SWUN-2/21/CH) prefusion F-trimeris produced by our Mammalian expression system and the target gene encoding Gln21-Leu508 is expressed with a 8His tag at the C-terminus. Some mutations are introduced to stabilize the trimeric prefusion state of BRSV Fusion glycoprotein.

Fusion glycoprotein F0; F

55.2 kDa

50-68 kDa, reducing conditions

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.

Bovine respiratory syncytial virus (BRSV) is a major contributor to respiratory disease in cattle worldwide. BRSV is an enveloped, non-segmented, negative-stranded RNA virus that belongs to the order Mononegavirales. The BRSV virion is made up of a lipid envelope generated from the host plasma membrane; it contains three virally encoded transmembrane surface glycoproteins that are arranged independently on the surface as spikes. These glycoproteins are the large glycoprotein G, the fusion protein F, and the small hydrophobic protein SH. The surface glycoproteins, the attachment (G) protein and the fusion (F) protein, are the major protective antigens of the virus. The F protein is a type I, membrane-bound, glycoprotein that mediates fusion of the viral membrane with that of the host cell to initiate a new infective cycle. The protein is synthesised as a single polypeptide, F0 precursor, that is post translationally cleaved into two subunits, that remain associated by disulphide bridges.