Taq DNA Polymerase(Cat. No.:E001)

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Price：

$60

SKU：

E001-01A

Size：

500U

500U×5

5000U

500U

500U×5

5000U

Count：

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Description

Taq DNA Polymerase is a thermostable recombinant DNA polymerase that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´ flap endonuclease activity. The enzyme lacks a 3´→5´exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an “A” overhang, which makes the enzyme ideal for TA cloning.
This product is obtained by expressing the Thermus aquaticus DNA Polymerase gene in E. coli and then purifying it several times. It is supplied with 5× Taq Buffer (with Mg²⁺), more ideal amplification results can be obtained.

Usage

Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

Features

High efficiency: With λDNA as template, the amplification length can reach 8kb, and the fragment of ≤4kb can be amplified efficiently.
High sensitivity: Target gene fragments can be amplified from 0.05ng human genomic DNA template.
High quality: fully meets the experimental requirements of Real Time PCR.
Uniquely formulated 5×Taq Buffer: makes the PCR amplification reaction more stable, sensitive and efficient.

Quality Control

After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection; qPCR method detection showed no residual E. coli DNA and no endonuclease or exonuclease contamination.

Storage

-20℃.

Components

Component	E001-01A	E001-01B	E001-01-M001	E001-02A	E001-02B	E001-02-M001
Taq DNA Polymerase (5U/μl)	100μl	100μl×5	1ml	100μl	100μl×5	1ml
dNTPs (10mM each)	200μl	200μl×5	1ml×2	-	-	-
5×Taq Buffer with Mg²⁺	1ml×2	1ml×10	20ml	1ml×2	1ml×10	20ml

Activity Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.

Notes

Template: Use of high quality, purified DNA templates greatly enhances the success of PCR.
Primers: Oligonucleotide primers are generally 20-40 nucleotides in length and ideally have a GC content of 40-60%. Higher concentrations may increase secondary priming and create spurious amplification products. Calculated melting temperatures (Tm) should be from 42-65°C.
Magnesium Concentration:
2 mM MgCl₂ is the most commonly used concentration for buffer preparation. It is also necessary to increase MgCl₂ concentrations when trying to compensate for DNA extracts containing PCR inhibitors since they also bind to Mg²⁺ ions and reduce their availability.
Too much MgCl₂: Excessive amounts of Magnesium Chloride in PCR reactions lead to non-specific binding of primers, resulting in errors in DNA replication.
Too little MgCl₂: In this situation, primers will fail to base pair with the DNA template, which results in a weak amplification or complete PCR failure.
Denaturation: Avoid longer or higher temperature incubations (unless required due to high-GC content of template).
Annealing: Typical annealing temperatures are 5°C below the lowest primer's Tm and often fall in the range of 50-60°C. Test higher annealing temperatures if spurious amplification products are observed. Typical annealing times are 15-30 seconds.
Preparation of Hot-Start Taq DNA polymerase: a monoclonal antibody against Taq DNA polymerase (Cat#: Z087) and Taq DNA polymerase are mixed in a 1:1 activity unit.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.

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