HotStart Taq DNA Polymerase (B)(Glycerol-Free)(Cat. No.:E097-03)

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Price：

$78

SKU：

E097-03A

Size：

250U

2500U

15KU

500U

5000U

Count：

Information

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Description

HotStar Taq DNA Polymerase (B) contains high-performance Taq DNA Polymerase bound to a proprietary antibody, and it is supplied in an inactive state and has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during PCR setup and the initial PCR cycle. Anti-Taq antibody is denatured in the initial DNA denaturation step of the PCR reaction, then the Taq DNA Polymerase activity is restored, so no additional hot start step is required. It has a 5´→3´ DNA polymerase activity and a 5´ flap endonuclease activity. The enzyme lacks a 3´→5´exonuclease activity (no proofreading ability). It leaves an “A” overhang, which makes the enzyme ideal for TA cloning.
HotStart Taq DNA Polymerase (B) (Glycerol-Free) is a glycerol free formulation of our regular HotStart Taq DNA Polymerase (B) and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. HotStart Taq DNA Polymerase (B) (Glycerol-Free) is available in different sizes and concentrations.

Usage

Highly specific PCR reaction.
Complex template amplification.
Multiplex PCR.
Genome amplification detection.
Fluorescence quantitative PCR.

Features

HotStart Taq DNA Polymerase (B) has high specificity and sensitivity, strong stability and wide application.
Glycerol-Free format available, suitable for robot-aided pipetting, automated high throughput testing, lyophilization.

Quality Control

After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection; qPCR method detection showed no residual E. coli DNA and no endonuclease or exonuclease contamination.

Storage

-20℃.

Components

Component	E097-03A Size：250U	E097-03B Size：2500U	E097-03-H30-U500 Size：15KU	E097-03-H50-U010 Size：500U	E097-03-H50-U100 Size：5000U
HotStart Taq DNA Polymerase (B) (Glycerol-Free, 5U/μl)	50μl	500μl	-	-	-
HotStart Taq DNA Polymerase (B) (Glycerol-Free, 30U/μl)	-	-	500μl	-	-
HotStart Taq DNA Polymerase (B) (Glycerol-Free, 50U/μl)	-	-	-	10μl	100μl
5×HS Taq Buffer with Mg²⁺*	1ml×2	10ml×2	-	2ml×2	-

Activity Definition

At 74°C, within 30 minutes, using activated salmon sperm DNA as a template primer, the amount of enzyme required to incorporate 10 nmol of deoxynucleotides into acid-insoluble substances is defined as one enzyme activity unit.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.

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