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Recombinant RNase Inhibitor (Murine)(Cat. No.:E125)
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SKU:
E125-01A
Size:
3000U
15KU
Count:
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1
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Description
  • Recombinant RNase Inhibitor (Murine) is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
  • Recombinant RNase Inhibitor (Murine) does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor. As a result, Recombinant RNase Inhibitor (Murine) has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Quality Control
After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection; qPCR method detection showed no residual E. coli DNA and no endonuclease or exonuclease contamination.
Storage
-20℃.
Components
ComponentE125-01A
(Size:3000U)
E125-01B
(Size:15KU)
Recombinant RNase Inhibitor (Murine, 40U/μl) 75μl375μl
Notes
  • Recombinant RNase Inhibitor (Murine) is active over a wide pH range (pH5.0-9.0), maximum activity at pH7.0-8.0.
  • Inactivation: Temperature higher than 65℃ or intense denaturation conditions for inactivation.
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For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.
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