
Description
2×LAMP Master Mix with Dye is designed to provide a simple, one-step solution for real-time detection of LAMP reactions via fluorescence. LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains all necessary components including enzyme mix, dNTPs, fluorescent dye and reaction buffer. Only primers and templates for isothermal amplification need to be added. Amplification results can usually be obtained within 30 minutes.

Usage
- Simple, one-step solution for Loop-Mediated Isothermal Amplification of DNA targets.
- Development of point-of-care testing devices and on-site testing reagents.

Quality Control
The detection showed no residual E. coli DNA and no endonuclease or exonuclease contamination.

Storage

Components
Component | E223-01A (Size:100rxns) | E223-01B (Size:500rxns) | 2×LAMP Master Mix with Dye | 1ml | 1ml×5 |

Notes
- This mix contains fluorescent dyes, which can be directly used for real-time quantitative reactions (reaction at 65℃, collecting fluorescence every 1min).
- The buffer contains Mg2+ at a concentration of 16 mM (Mg2+ final concentration is 8 mM).
- The reaction temperature and time can be adjusted according to the specific experimental template amount.
For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.
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