BenzoNuclease®, GMP Grade(Cat. No.:GMP-1707)

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Price：

$630

SKU：

GMP-1707-100KU

Size：

100KU

200KU

5000KU

Count：

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Description

BenzoNuclease® is a recombinant endonuclease derived from Serratia marcescensa. The protein is expressed in Escherichia coli (E. coli). BenzoNuclease® has been shown to exert a broad spectrum of substrate specificity to degrade both DNA and RNA—whether single-stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, BenzoNuclease® hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate-terminated oligonucleotides, which are three to five bases in length.
In scientific research, BenzoNuclease® can be used as the preferred enzyme preparation for supernatant of cultured cells and viscosity removal of cell lysate, which can remove nucleic acid interference and improve the subsequent protein purification or functional research. At the same time in the vaccine industry, protein and polysaccharide pharmaceutical industry, used to remove the host residual nucleic acid, greatly reduce the vaccine and protein products of nucleic acid pollution, reduce the processing steps, improve the yield of products.
This product is expressed by large-scale fermentation of E. coli, and is produced with raw materials of medicinal specifications. The host protein residue, nucleic acid residue and common pathogens are strictly controlled, and the production and quality management procedures of the product comply with GMP regulations to ensure the traceability of the production process and all raw materials.
FDA DMF

Usage

Used to remove exogenous nucleic acid from vaccine products, reduce the risk of residual toxicity of nucleic acid and improve product safety.
Used to reduce the viscosity of feed liquid caused by nucleic acid, shorten the processing time and increase the protein yield.
Used to remove nucleic acid winding on the surface of particles (viruses, inclusion bodies, etc.), which is conducive to the release and purification of particles.
Used to prepare samples for ELISA, column chromatography, 2D electrophoresis and Western blot analysis. The resolution and recovery can be improved after BenzoNuclease® treatment.
Used to prevent cell clumping.

Quality Control

Element	Standard
Appearance	clear, transparent solution
Visible Particles	meet the specification
pH	7.5-8.5
Activity	250-400 U/μl
Purity	≥99%
Spec. Activity	≥1.1×10⁶ U/mg
Protease Activity	no protease activity detectable
Bacterial Endotoxins	< 0.01 EU/KU
Host-cell Protein Residues	≤0.005%
Sterility	meet the specification
Mycoplasma	negative
Heavy Metal Residue	≤ 10 ppm

Storage

-20±5℃

Components

SKU	Size	Item	Quantity
GMP-1707-100KU	100KU	BenzoNuclease®, GMP Grade (250U/μl)	400μl
GMP-1707-200KU	200KU	BenzoNuclease®, GMP Grade (250U/μl)	800μl
GMP-1707-5000KU	5000KU	BenzoNuclease®, GMP Grade (250U/μl)	20ml

Activity Definition

In a 2.625 mL reaction system at 37℃ and pH 8.0, one unit of BenzoNuclease® is defined as the amount of enzyme that causes a change in absorbance at 260 nm of 1.0 absorption units within 30 minutes.

Recommendations

Reference Dosage:

Usage scenarios	Preparation of electrophoretic protein samples	Protein production	Production of vaccines and viruses	Cell medicine
Number of cells	1×106 cells (10mg tissue)	1g wet weight (10ml resuspension)	1L fermentation supernatant	1L cultures
Minimum dosage	125 U	250 U	100 U	100U
Recommended dosage	500 U	2500 U	25000 U	5000U

Time: Normally the action time is 37℃ for 15~60 min, 25℃ for 30~120 min.
Reaction Condition:

Condition	Optimal*	Effective**
Mg²⁺	1-2mM	1-10mM
pH	8.0-9.2	6.0-10.0
Temperature	37℃	0-42℃
DTT	0-100mM	>100mM
BME	0-100mM	>100mM
Na⁺, K⁺, etc.	0-20mM	0-150mM
PO₄^3-	0-10mM	0-100mM

*: Optimal is defined as the condition under which BenzoNuclease® retains >90% of its activity.
**: Effective is defined as the condition under which BenzoNuclease® retains >15% of its activity.

Notes

For sample repackaging, please use sterile pyrogen-free bottles with medical device certificates. Generally, Eppendorf tubes on the market are not medical devices.
The formulations of Novoprotein products have been optimized, and generally samples stored at 4°C for two weeks do not affect their biological activity, but long-term storage at 4°C is not recommended.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.

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