RNase Inhibitor, GMP Grade(Cat. No.:GMP-E125-HC)

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SKU：

GMP-E125-HC-U100

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100KU

1000KU

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Description

RNase Inhibitor inhibits RNase A, B and C with high efficiency by forming a 1:1 complex with them. The characteristics of RNase Inhibitor are similar to those derived from pig liver and human placenta. The inhibition is reversible, as the complex can be dissociated by urea and sulfhydryl reagents in which RNase refolds and the inhibitor is inactivated. It can be directly added to the reaction containing RNA. Different from other competitive inhibitors (e.g., nucleic acids, inorganic phosphoric acids), this product is a protein, which can be easily removed from the reaction by phenol extraction. However, the reverse transcriptase RNase H could not be inhibited.
Recombinant RNase Inhibitor is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
This product has completed the DMF record of FDA and passed the HALAL certification.

Quality Control

Element	Standard
Appearance	Clear and transparent solution
Identification	Positive
Visible Particles	Meet the specification
pH	7.0-8.0
Activity	980KU/ml-1020KU/ml
Purity	≥95%
Endonuclease Residues	The degradation of substrate was ≤10%
Exonuclease Residues	The degradation of substrate was ≤10%
RNase Residues	The degradation of substrate was ≤10%
Bacterial Endotoxins	＜10EU/mg
Exogenous DNA Residues	≤100pg/mg
Host-cell Protein Residues	≤50ppm
Mycoplasma	Negative
Heavy Metals	≤10ppm
Microbial Limit	Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml

Activity Definition

One unit is defined as the amount of Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.

Components

SKU	Components	Volume
GMP-E125-HC-U100	RNase Inhibitor, GMP Grade (1000U/μl)	100μl
GMP-E125-HC-M001	RNase Inhibitor, GMP Grade (1000U/μl)	1ml

Storage

At -20±5℃.

Usage

1. Synthesis of cDNA
2. In-vitro translation
3. Separation of polyribosomes
4. In-vitro transcription

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