T4 RNA Ligase 2, GMP Grade(Cat. No.:GMP-M050)

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GMP-M050-U100

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Description

T4 RNA Ligase 2 is an ATP-dependent double-strand RNA ligase (dsRNA ligase). It can be used for intramolecular cyclic ligation and intermolecular linear ligation of double-stranded RNA, and can also be used for intramolecular or intermolecular RNA ligation of double-stranded nucleic acids (RNA duplex, RNA/DNA hybrid strand, or DNA duplex) for the 3' hydroxyl group of the RNA strand and the 5' phosphate group of the DNA strand. T4 RNA Ligase 2 requires the presence of 5' phosphate and 3' hydroxyl groups for ligation, which can be a ligation reaction between the 5' phosphate group present in the RNA strand or DNA strand and the 3' hydroxyl group present in the RNA strand. Treatment at 65 °C for 15 min or boiling for 2 min can inactivate the enzyme.
This product is produced with pharmaceutical specifications of raw and auxiliary materials, and strictly controls all kinds of pollution in the production process, etc., in line with GMP standard product production and quality management procedures, to ensure that the production process and all raw and auxiliary materials can be traced.

Quality Control

Element	Standard
Appearance	Clear and transparent solution
Identification	Positive
Visible Particles	Meet the specification
pH	7.5±0.2
Activity	8.0U/ml-12.0U/ml
Purity	≥95%
Endonuclease Residues	The degradation of substrate was ≤10%
Exonuclease Residues	The degradation of substrate was ≤10%
RNase Residues	The degradation of substrate was ≤10%
Bacterial Endotoxins	＜5EU/ml
Exogenous DNA Residues	≤100pg/mg
Host-cell Protein Residues	≤50ppm
Mycoplasma	Negative
Heavy Metals	≤10ppm
Microbial Limit	Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml

Storage

-20℃ ± 5 ℃

Components

Notes

For longer and more complex oligonucleotides, incubation at 25 °C for 2 h and then overnight at 16 °C may increase yield;
The enzyme is an ATP-dependent RNA ligase.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.

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